| feature | ||||||
|---|---|---|---|---|---|---|
| General summary | H9_2_4 | H9_3_4 | H9_4_4 | HEYA8_1_4 | HEYA8_2_4 | HEYA8_3_4 |
| Mean read length | 1,204.2 | 1,193.0 | 1,207.6 | 1,134.2 | 1,120.8 | 1,133.2 |
| Mean read quality | 11.7 | 11.7 | 11.7 | 11.7 | 11.7 | 11.6 |
| Median read length | 947.0 | 938.0 | 950.0 | 899.0 | 888.0 | 894.0 |
| Median read quality | 12.3 | 12.3 | 12.3 | 12.3 | 12.3 | 12.3 |
| Number of reads | 1,585,284.0 | 1,671,014.0 | 1,498,136.0 | 1,755,040.0 | 1,725,141.0 | 1,566,988.0 |
| Read length N50 | 1,364.0 | 1,337.0 | 1,370.0 | 1,246.0 | 1,219.0 | 1,242.0 |
| STDEV read length | 757.2 | 744.1 | 758.0 | 684.4 | 676.5 | 695.3 |
| Total bases | 1,909,030,620.0 | 1,993,457,234.0 | 1,809,079,717.0 | 1,990,492,983.0 | 1,933,576,845.0 | 1,775,698,192.0 |
| Number, percentage and megabases of reads above quality cutoffs | ||||||
| >Q10 | 1585284 (100.0%) 1909.0Mb | 1671014 (100.0%) 1993.5Mb | 1498136 (100.0%) 1809.1Mb | 1755040 (100.0%) 1990.5Mb | 1725141 (100.0%) 1933.6Mb | 1566987 (100.0%) 1775.7Mb |
| >Q15 | 58031 (3.7%) 98.8Mb | 56814 (3.4%) 97.9Mb | 52416 (3.5%) 90.5Mb | 55952 (3.2%) 91.2Mb | 58025 (3.4%) 91.9Mb | 47663 (3.0%) 79.3Mb |
| >Q20 | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 3 (0.0%) 0.0Mb | 1 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb |
| >Q25 | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb |
| >Q30 | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb |
| Top 5 highest mean basecall quality scores and their read lengths | ||||||
| 1 | 19.8 (752) | 19.7 (599) | 19.6 (633) | 21.4 (513) | 20.2 (575) | 19.5 (562) |
| 2 | 19.1 (558) | 19.1 (850) | 19.1 (695) | 20.5 (975) | 19.8 (1350) | 19.4 (510) |
| 3 | 19.0 (765) | 18.8 (3756) | 19.1 (5211) | 20.2 (544) | 19.6 (782) | 18.9 (854) |
| 4 | 18.9 (2055) | 18.8 (561) | 19.1 (1315) | 19.1 (2038) | 19.2 (611) | 18.7 (1004) |
| 5 | 18.7 (710) | 18.8 (504) | 19.0 (518) | 19.1 (631) | 18.9 (1610) | 18.7 (3870) |
| Top 5 longest reads and their mean basecall quality score | ||||||
| 1 | 12885 (13.0) | 12692 (14.1) | 14412 (12.6) | 13448 (11.9) | 14254 (12.1) | 13541 (13.6) |
| 2 | 12561 (13.3) | 12337 (13.0) | 12264 (12.2) | 11633 (10.4) | 12702 (11.6) | 11263 (12.1) |
| 3 | 12410 (15.4) | 11524 (13.2) | 12235 (11.6) | 11275 (14.8) | 12076 (12.8) | 11017 (15.1) |
| 4 | 11911 (14.4) | 11291 (13.6) | 12183 (12.4) | 11145 (10.3) | 11870 (12.2) | 10941 (13.4) |
| 5 | 11184 (14.4) | 11195 (13.0) | 11761 (15.0) | 10940 (14.6) | 11590 (12.8) | 10689 (12.5) |